ABSTRACT
Microorganisms are important components in the structure and function of
organic matter in soil, hence this work was done to identify bacterial communities
present in flooded soil. Soil samples were collected from eight different areas
within Benin metropolis, Edo State, Nigeria. The samples were cultured on using
pour plate method, identification of the main bacterial groups was carried out
based on cultural, morphology and biochemical test. The results obtained showed
the total heterotrophic bacterial counts ranging from 2.8×103
to 2.0×104 cfu/g. A
total of six (6) organisms were isolated and they include Escherichia coli,
Staphylococcus aureus, Bacillus megaterium, Micrococcus luteus, Pseudomonas
fluorescens and Acinetobacter species. Bacillus megaterium was observed as the
most prevalent with (87.5%), while Staphylococcus aureus had the least
occurrence (37%). The prevalence of these microbes in the soil is of public health
concern because they create an avenue for transmission of opportunistic infections.
TABLE OF CONTENT
CERTIFICATION……………………………………………………………………………………………..i
APPROVAL…………………………………………………………………………………………………… ii
DEDICATION………………………………………………………………………………………………… iii
ACKNOWLEDGEMENT………………………………………………………………………………….. vi
TABLE OF CONTENT……………………………………………………………………………………… v
LIST OF TABLES…………………………………………………………………………………………….. vi
ABSTRACT……………………………………………………………………………………………………. vii
CHAPTER ONE
1.0 INTRODUCTION………………………………………………………………………………………. 1
1.1 AIM AND OBJECTIVES……………………………………………………………………………… 5
CHAPTER TWO
2.0 LITERATURE REVIEW………………………………………………………………………………… 6
CHAPTER THREE
3.0 MATERIALS AND METHODS…………………………………………..………………………… 23
CHAPTER FOUR
4.0 RESULTS………………………………………………………………………………………………... 28
CHAPTER FIVE
5.0 DISCUSSION…………………………………………………………………………………………… 35
5.1 CONCLUSION…………………………………………………………………………………………. 39
REFERENCES…………………………………………………………………………………………………….. 40
APPENDIX A……………………………………………………………………………………………………… 44
APPENDIX B……………………………………………………………………………………………………… 47
